Youtube daily la Sep 23 2017

Hi everyone, today we're going to do a bathroom tour.

So, let's go.

So, when we enter the bathroom, the first thing that we see is the vanity.

The vanity is in a shade of brown.

With a lot of storage below.

The walls are painted in grey in the bathroom.

Now, let's talk about what we see on the vanity.

So, on the vanity we have a small white bathroom sink.

Beside it we have a bottle of soap to wash hands.

And not so far we have a towel holder.

It's a DIY, that I did myself which cost me about 4$.

Basically, it's just a towel holder from the dollar store on which I glued mirrors and diamond wicks around it.

Here is the mirror of the bathroom.

The frame of the mirror is also a DIY, because the original mirror was dirty.

And we installed a frame ourselves with pieces of MDF that we cut.

And we spray painted the frame with a silver grey. I don't know if we can see it well.

It's prettier this way. The fabrication of the frame plus the spray paint cost us around 15$.

On the vanity, we also have a tray which contains all the necessary for our guests.

We have a few bottles of cream, some wipes, a toothbrush, a toothpaste and many other things.

I did not buy this little box, I had it in my house and just recycled it. I just glued some diamond wicks to it.

The tray I bought it at Winners, I don't remember the price but it wasn't more than 20$.

It's a tray with 2 stages, it's practicle because it doesn't take much space.

On the walls, we have frames.

We were looking for something in the grey color to complete the decoration but we did not find anything.

So, we bought frames at the dollar store. Each frame was about 2$.

And we printed ourselves some verses.

Always in the shades of grey and white. The colors of the bathroom.

We have some toilet paper, in a vase made of glass. All the additional toilet paper is there.

Here we have some shelves.

On those shelves there are a few decorations.

Here we have a pot with some greenery. I bought the pot at Ikea for 2$ or 3$ and the greenery that's inside was 2$.

The total amount of this was 5$ to 6$.

Here we have some leaves. I generally use them for the Christmas decorations.

But, I thought that it was looking good here. So, it's some silver leaves that I bought at the dollar store.

It was maybe 1.50$.

This decorative element is a vase with mirrors. It's a DIY. I bought the mirrors at the dollar store.

So, 4 mirrors and some silver diamond wicks to make it prettier. And also some flowers that I bought at the dollar store.

The total for this vase is around 7$ to 8$.

This shelf also allow us to put a few extra towels on there.

Here we also have a few decorative elements that are very useful.

We have some tissues for the nose. The tissues are in this vase that is also a DIY. It's a simple vase bought at the dollar store around 2$.

I decorated it with some pearls and diamonds.

I removed the tissues from the package and put them in the vase. It's prettier.

Here I have my vase which contains my cleaning bombs.

It's almost empty, many were already used. It is also a DIY. At first, it was just an empty vase then I added some diamonds.

The cleaning bombs are also a DIY. They are made of baking soda, essential oils and some acid.

Just for cleaning.

Here we have some towels, I added a white and grey mesh. Again just to make it pretty.

This was our bathroom tour. I hope that you liked the video.

For more infomation >> BATHROOM TOUR | HOME DECOR | TOUR DE LA SALLE D'EAU | Akoy Family - Duration: 8:01.

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Sampdoria-Milan: la conferenza stampa di Giampaolo - Duration: 3:59.

We're back at home, in front of our own fans

and I imagine the supporters are pleased as well

because they've travelled a lot to support us.

I will pick the best possible side for this match.

Those who needed a rest have had one.

Those who I needed to protect from potential knocks and injuries have been kept fit.

So I will start with the best 11 for this match who,

from a physical, technical, tactical and mental perspective,

are able to withstand a tough match against good opposition.

We're going to try and compete, just like I said a fortnight ago.

AC Milan have a very clearly defined game model.

They're technically very good and keep the ball well.

We'll have to produce a great performance and go that extra mile

to go toe to toe with a quality AC Milan team who move the ball very well.

They're the side with the second best ball possession stats in the league after Napoli.

They're a team that tend to stretch you and make you run long distances.

We need to be aware of where AC Milan's strengths lie.

It's a tough match in terms of combatting what the opposition are good at.

We need to do outdo ourselves.

My staff and I have watched AC Milan three or four times including their European match.

I know what AC Milan are all about, I'm aware of how they play.

They often play with 12 men on the pitch because they use their keeper a lot.

As I said, they stretch you and make you run long distances.

They use the goalkeeper to hog ball possession.

I know all about AC Milan.

I know what's in store for us and I think the lads realise how we must play

and now all we have to do is get out there.

On top of the tactical aspects during the game, there are other factors

like heart, desire, passion and excitement.

Plus our fans can always give us a lift.

A number of factors come into play during a football match.

My aim is to make my side competitive regardless of the opposition.

That means we never go into games thinking we're beaten.

The league table will show what we can do now,

what we'll be like in two months and where we'll be come May.

I don't know. If the president says we must be the best of the rest outside the top six

and we're able to achieve that, the president will be right.

If we don't achieve that, I'll have got something wrong in the president's eyes.

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Reconstrucción Cerebral En Placasurgimiento De La Neuroinflamaciónde Retrotransposones - Duration: 7:26.

UCTV, UNIVERSITY OF CALIFORNIA TELEVISION - WWW.UCTV.TV

UC SAN DIEGO STEM CELL PROGRAM

RESEARCH ADVANCES

RECONSTRUCTING THE BRAIN IN A DISH

EMERGENCE OF NEUROINFLAMMATION FROM RETROTRANSPOSONS

In this work we took advantage of a rare disorder

like Aicardi-Goutieres to really explore the fundamental mechanism

that happens in your brain.

Clinically you can see the manifestation

pre-birth by ultrasound.

You can detect that their brains are not developing well.

They have microcephalic brains.

Overall, it's a very severe neurological disorder.

DESIGNING THE STUDY AND CHOOSING TARGETS FOR INVESTIGATION

There are a couple of things that we knew about Aicardi-Goutieres.

One is the fact that it behaves like an autoimmune response

in the absence of any obvious infection.

Second is the nature of the genetic mutations

in genes such as TREX1.

And finally, the complete absence of an animal model,

they don't show the neurological abnormalities

that we see in those patients.

STUDY TARGETS: TREX1 AND LINE1 ELEMENTS

TREX1 stands for three prime repair exonuclease 1.

Exonuclease in general, they degrade RNA or DNA,

so the body can defend against a virus.

So TREX1 basically its function is to degrade DNA,

single-stranded DNA.

If you look at TREX1 inside the cells,

we see an upregulation of repetitive regions of the genome,

including retrotransposons such as line retrotransposons.

Line 1 elements are repetitive sequences

that are able to move from one region of the genome into another

by a mechanism called retrotransposition,

and the way that they do that is by using reverse transcriptase.

As far as we know, Line 1 elements are active during early development

and also in the brain.

CHOOSING A METHODOLOGY: STEM CELLS

With the absence of a good animal model,

the only way to study this condition in a human background

is to take advantage of human pluripotent stem cells.

So we use IPSCs, induced pluripotent stem cells,

and embryonic stem cells.

And you create specific mutations and use these cells

to differentiate specific cell lines.

Using these cell lines

we are able to create a different model,

an organoid, that recapitulates

the process that we have in our brain development.

MODEL FUNCTION AND OBSERVED EFFECTS

So when we engineered the cells

we confirmed that the mutation is there

but there was no TREX1 protein inside those cells.

In a healthy cell you always have your DNA,

in the nucleus, where it's supposed to be.

And so what we saw is that outside of the nucleus

to an extreme amount, single-stranded DNA existing.

And a lot of the DNA was actually the Line 1 retrotransposon element.

They behaved like normal cells

with the TREX1 protein being expressed.

The big surprise came when we pushed or instructed

these neuro progenitor cells to become neurons,

and we see a dramatic effect.

By a large proportion, the AGS patient cells were just dying.

We knew then that it was a problem of cell-death.

The TREX1 mutant cells virtually cannot make neurons.

That strong dramatic phenotype prompted us to make astrocytes.

And when we compare the amount of Line retrotransposons

we realized that there were way more

in the cytoplasm compared to controls.

What we see is that these cells look like immunoreactive.

They feel like they were attacked by a virus or something, we think.

They basically sense retrotransposons as the enemy

and they trigger this interferon response.

The astrocyte signals, they were toxic.

So AGS patients have intrinsic toxicity

within the neuron itself,

and extrinsic toxicity from the astrocytes.

So they're getting hit twice.

EFFECTS ON ORGANOIDS

In the 3D system the effects are even more dramatic.

The main thing we saw on the AGS side

is that they were dying as fast as they were growing.

And as a consequence we have cerebral organoids

that looks like microcephalic.

They're smaller in size and the amount of cell death

that we see in those neurons

is elevated compared to the control cells.

THERAPEUTIC OPPORTUNITIES:

REVERSE TRANSCRIPTION AND ENDOGENOUS CELL TOXICITY

Once we have these dramatic effects

we thought about a potential therapeutic intervention.

So there are actually reverse transcriptase drugs.

So we tried these same drugs.

When you added these retrovirals,

there was much less single-stranded DNA in the cytocell,

and then we saw that they were much healthier.

So they didn't have the toxicity.

They didn't have the interferon response in astrocytes.

We completely rescued the cell death.

Virtually they become indistinguishable from the controls.

In the brain organoids it works as nice as in the 2D system.

The retroviral drugs could actually make these brain organoids

thrive as much as in the control cells.

THERAPEUTIC OPPORTUNITIES: CONTROLLING INTERFERON RESPONSE

Essentially astrocytes are secreting interferons

that are killing neurons.

If you could block this pathway

you could prevent the extrinsic cell death.

To do that we actually use a drug

that can block the interferon receptor,

and by doing that, we could see the same effects.

We rescue the amount of neuronal death

that we see in the mutant cells

to a level that's comparable to the control cells.

CONCLUSIONS AND IMPLICATIONS OF THE STUDY

The conclusions of our work is that we could actually

nail down the exact mechanism

both at the molecular and the cellular level

of what's going on during the neurodevelopment

of Aicardi-Goutieres syndrome.

So we are definitely excited with the idea that one can design

a clinical trial to treat Aicardi-Goutieres.

But it's also possible that this mechanism

is more fundamental than we think,

and it might actually be working or contributing to other diseases

such as autism, aging, or neurodegenerative disorders

where Line elements are highly expressed in their brains.

So it might be more common that we think.

RECONSTRUCTING THE BRAIN IN A DISH

EMERGENCE OF NEUROINFLAMMATION FROM RETROTRANSPOSONS

A VIDEO SUMMARY OF MODELING OF TREX1-DEPENDENT

AUTOIMMUNE DISEASE USING HUMAN STEM CELLS

HIGHLIGHTS L1 ACCUMULATION AS A SOURCE OF NEUROINFLAMMATION

PUBLISHED IN CELL STEM CELL, AUGUST 2017

AUTHORS

THE VIEWS, CONTENTS AND OPINIONS EXPRESSED HEREIN

DO NOT NECESSARILY REPRESENT THOSE OF THE UNIVERSITY OF CALIFORNIA

For more infomation >> Reconstrucción Cerebral En Placasurgimiento De La Neuroinflamaciónde Retrotransposones - Duration: 7:26.

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